|
| Status |
Public on Sep 30, 2008 |
| Title |
SCCmec mutant, stationary phase, biological rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
SCCmec mutant - sta rep3
|
| Organism |
Staphylococcus aureus |
| Characteristics |
SF8300
|
| Treatment protocol |
samples were untreated
|
| Growth protocol |
For microarray experiments, overnight cultures were diluted into 30 ml of TSB and incubated at 37C with shaking until grown to exponential or stationary growth phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by use of a Bio101 kit (Vista, CA) by the standard protocol with minor modification (e.g. use of Trizol reagent instead of RNApro solution).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from total RNA (Affymetrix Prokaryotic Sample and Array Processing Protocol, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cDNA were hybridized for 16 hr at 45C on a commercially available array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000.
|
| Description |
Gene expression data from_SCCmec mutant, stationary phase, biological rep 3
|
| Data processing |
The data were analyzed with GCOS 1.4 using Affymetrix default analysis and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Oct 12, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Amy M Palazzolo-Ballance |
| E-mail(s) |
ballancea@niaid.nih.gov
|
| Phone |
406-363-9349
|
| Organization name |
NIH/NIAID
|
| Department |
Rocky Mountain Laboratory
|
| Lab |
Laboratory of Human Bacterial Pathogenesis
|
| Street address |
903 S. 4th Street
|
| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
| |
|
| Platform ID |
GPL1339 |
| Series (1) |
| GSE9308 |
The ACME and SCCmec Linkage of Virulence with Resistance in the Community Methicillin Resistant S. aureus USA300 Clone |
|
