|
| Status |
Public on Jan 13, 2008 |
| Title |
NA12005 growth 3. |
| Sample type |
RNA |
| |
|
| Source name |
CEU NA12005 Biological Growth 3
|
| Organism |
Homo sapiens |
| Characteristics |
Gender: male, Cell-type: lymphoblasts
GM12005_03
Coriell cell line repository identifier:GM12005
|
| Biomaterial provider |
Coriell Cell Repositories
http://ccr.coriell.org/Sections/Search/Search.aspx?PgId=165&q=GM12005
|
| Treatment protocol |
Cells were harvested at a density of 0.8-1.1 x 10e6 cells/mL. Cells were then resuspended and lysed in TRIzol reagent
|
| Growth protocol |
Cells were grown at 37°C and 5% CO2 in RPMI 1640 medium supplemented with 15% heat-inactivated fetal bovine serum, 2 mM L-glutamine and penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinlabelled target for the microarray experiment were prepared using 1 ug of total RNA. The RNA was subjected to a rRNA removal procedure with the RiboMinus Human/Mouse Transcriptome Isolation Kit (Invitrogen) and cDNA was synthesized using the GeneChip® WT (Whole Transcript) Sense Target Labeling and Control Reagents kit as described by the manufacturer (Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 ug of cRNA were hybridized for 16-20 hr at 45C on GeneChip Human Exon 1.0 ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Probeset expression data from NA12005 growth 3
|
| Data processing |
The data were analyzed with Affymetrix Power Tools package using Affymetrix default analysis settings and sketch quantile as normalization method.
|
| |
|
| Submission date |
Oct 18, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Tony Kwan |
| E-mail(s) |
tony.kwan@mcgill.ca
|
| Phone |
514-398-3311
|
| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Street address |
740 Dr. Penfield
|
| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H3A 0G1 |
| Country |
Canada |
| |
|
| Platform ID |
GPL5175 |
| Series (1) |
| GSE9372 |
Genome-wide analysis of transcript isoform variation in humans |
|
