|
Status |
Public on Jun 10, 2017 |
Title |
milk somatic cells Polish Fawn Improved goat_1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
milk somatic cells (MSC)
|
Organism |
Capra hircus |
Characteristics |
breed: Polish Fawn Sex: female tissue: milk somatic cells
|
Treatment protocol |
MSC and FMC was protected with Qiazol reagent from the RNeasy Lipid Tissue Mini Kit Qiagen (Qiagen, USA); Blood from vein was mixed with erythrocyte lysis buffer RBCL and protected with fenozol from Total RNA mini Kit (A & A Biotechnology,POLAND)
|
Extracted molecule |
total RNA |
Extraction protocol |
isolation of total RNA from goat MSC and MFG the RNeasy Lipid Tissue Mini Kit (Qiagen, USA); the RNA isolationfrom goat PNCB was conducted by using Total RNA mini Kit (A & A Biotechnology, POLAND)
|
Label |
Cy5
|
Label protocol |
according to the manufacturer's protocol
|
|
|
Channel 2 |
Source name |
common reference sample composed of 7 healthy goats
|
Organism |
Capra hircus |
Characteristics |
tissue: peripheral nuclear blood cells breed: Polish breeds (PWI=4 & PFI=3) lactation stage: 4th
|
Treatment protocol |
MSC and FMC was protected with Qiazol reagent from the RNeasy Lipid Tissue Mini Kit Qiagen (Qiagen, USA); Blood from vein was mixed with erythrocyte lysis buffer RBCL and protected with fenozol from Total RNA mini Kit (A & A Biotechnology,POLAND)
|
Extracted molecule |
total RNA |
Extraction protocol |
isolation of total RNA from goat MSC and MFG the RNeasy Lipid Tissue Mini Kit (Qiagen, USA); the RNA isolationfrom goat PNCB was conducted by using Total RNA mini Kit (A & A Biotechnology, POLAND)
|
Label |
Cy3
|
Label protocol |
according to the manufacturer's protocol
|
|
|
|
Hybridization protocol |
according to the manufacturer's protocol
|
Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
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|
|
Submission date |
Nov 09, 2016 |
Last update date |
Jun 10, 2017 |
Contact name |
Joanna Pławińska-Czarnak |
E-mail(s) |
jp-cz@pulse.pl
|
Organization name |
WULS-SGGW
|
Street address |
Nowoursynowska 159
|
City |
Warszawa |
ZIP/Postal code |
02-787 |
Country |
Poland |
|
|
Platform ID |
GPL11648 |
Series (1) |
GSE89693 |
Selected tissues of two Polish goat breeds do not differ on genomic level |
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