|
| Status |
Public on Jun 10, 2017 |
| Title |
peripheral nuclear cells blood Polish Fawn Improved goat_3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
peripheral nuclear cells blood (PNCB)
|
| Organism |
Capra hircus |
| Characteristics |
breed: Polish Fawn
Sex: female
tissue: peripheral nuclear blood cells
|
| Treatment protocol |
MSC and FMC was protected with Qiazol reagent from the RNeasy Lipid Tissue Mini Kit Qiagen (Qiagen, USA); Blood from vein was mixed with erythrocyte lysis buffer RBCL and protected with fenozol from Total RNA mini Kit (A & A Biotechnology,POLAND)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
isolation of total RNA from goat MSC and MFG the RNeasy Lipid Tissue Mini Kit (Qiagen, USA); the RNA isolationfrom goat PNCB was conducted by using Total RNA mini Kit (A & A Biotechnology, POLAND)
|
| Label |
Cy5
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| Channel 2 |
| Source name |
common reference sample composed of 7 healthy goats
|
| Organism |
Capra hircus |
| Characteristics |
tissue: peripheral nuclear blood cells
breed: Polish breeds (PWI=4 & PFI=3)
lactation stage: 4th
|
| Treatment protocol |
MSC and FMC was protected with Qiazol reagent from the RNeasy Lipid Tissue Mini Kit Qiagen (Qiagen, USA); Blood from vein was mixed with erythrocyte lysis buffer RBCL and protected with fenozol from Total RNA mini Kit (A & A Biotechnology,POLAND)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
isolation of total RNA from goat MSC and MFG the RNeasy Lipid Tissue Mini Kit (Qiagen, USA); the RNA isolationfrom goat PNCB was conducted by using Total RNA mini Kit (A & A Biotechnology, POLAND)
|
| Label |
Cy3
|
| Label protocol |
according to the manufacturer's protocol
|
| |
|
| |
| Hybridization protocol |
according to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Nov 09, 2016 |
| Last update date |
Jun 10, 2017 |
| Contact name |
Joanna Pławińska-Czarnak |
| E-mail(s) |
jp-cz@pulse.pl
|
| Organization name |
WULS-SGGW
|
| Street address |
Nowoursynowska 159
|
| City |
Warszawa |
| ZIP/Postal code |
02-787 |
| Country |
Poland |
| |
|
| Platform ID |
GPL11648 |
| Series (1) |
| GSE89693 |
Selected tissues of two Polish goat breeds do not differ on genomic level |
|
