|
Status |
Public on Apr 09, 2008 |
Title |
kernel_development_5DAP_to_1DAP_rep1_A |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
5DAP
|
Organism |
Zea mays |
Characteristics |
Zea mays, Kernel, Development
|
Growth protocol |
Natural conditions, growing season begins from May to September, on the China Agricultural University research farm in Beijing, China.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a modified hot phenol method.
|
Label |
Cy5
|
Label protocol |
Cy5-cDNA were prepared according to the recommendations of the Maize Oligo Array Project (http://www.maizearray.org/).
|
|
|
Channel 2 |
Source name |
1DAP
|
Organism |
Zea mays |
Characteristics |
Zea mays, Kernel, Development
|
Growth protocol |
Natural conditions, growing season begins from May to September, on the China Agricultural University research farm in Beijing, China.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using a modified hot phenol method.
|
Label |
Cy3
|
Label protocol |
Cy3-cDNA were prepared according to the recommendations of the Maize Oligo Array Project (http://www.maizearray.org/).
|
|
|
|
Hybridization protocol |
Hybridization and post hybridization wash were performed according to the protocal of the Maize Oligo Array Project (http://www.maizearray.org/).
|
Scan protocol |
Hybridized slides were scanned by a Genepix 4000B (Axon, USA).
|
Description |
Gene expression data from maize kernel
|
Data processing |
Raw data from microarray hybridization, exported from GenePix suites 6.0 (Axon, USA), was imported into LIMMA with annotation and spot types. Spots with a negative flag value were assigned a weight of 0.1 in the subsequent analysis. Background-subtracted signal intensities were normalized using a two-step normalization: print-tip group loss (within-array normalization) and then between-array scale normalization.
|
|
|
Submission date |
Oct 19, 2007 |
Last update date |
Apr 09, 2008 |
Contact name |
Guoying Wang |
E-mail(s) |
gywang@caas.net.cn
|
Phone |
86-10-62162927
|
Organization name |
Chinese Academy of Agricultural Sciences
|
Department |
Institute of Crop Sciences
|
Lab |
Maize Molecular Biology
|
Street address |
Zhongguancun Nandajie 12
|
City |
Beijing |
ZIP/Postal code |
100081 |
Country |
China |
|
|
Platform ID |
GPL5439 |
Series (1) |
GSE9386 |
Genome-wide analysis of gene expression profiles during the kernel development of maize (Zea mays L.) |
|