|
| Status |
Public on Apr 09, 2008 |
| Title |
kernel_development_25DAP_to_15DAP_rep2_B |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
25DAP
|
| Organism |
Zea mays |
| Characteristics |
Zea mays, Kernel, Development
|
| Growth protocol |
Natural conditions, growing season begins from May to September, on the China Agricultural University research farm in Beijing, China.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a modified hot phenol method.
|
| Label |
Cy5
|
| Label protocol |
Cy5-cDNA were prepared according to the recommendations of the Maize Oligo Array Project (http://www.maizearray.org/).
|
| |
|
| Channel 2 |
| Source name |
15DAP
|
| Organism |
Zea mays |
| Characteristics |
Zea mays, Kernel, Development
|
| Growth protocol |
Natural conditions, growing season begins from May to September, on the China Agricultural University research farm in Beijing, China.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a modified hot phenol method.
|
| Label |
Cy3
|
| Label protocol |
Cy3-cDNA were prepared according to the recommendations of the Maize Oligo Array Project (http://www.maizearray.org/).
|
| |
|
| |
| Hybridization protocol |
Hybridization and post hybridization wash were performed according to the protocal of the Maize Oligo Array Project (http://www.maizearray.org/).
|
| Scan protocol |
Hybridized slides were scanned by a Genepix 4000B (Axon, USA).
|
| Description |
Gene expression data from maize kernel
|
| Data processing |
Raw data from microarray hybridization, exported from GenePix suites 6.0 (Axon, USA), was imported into LIMMA with annotation and spot types. Spots with a negative flag value were assigned a weight of 0.1 in the subsequent analysis. Background-subtracted signal intensities were normalized using a two-step normalization: print-tip group loss (within-array normalization) and then between-array scale normalization.
|
| |
|
| Submission date |
Oct 20, 2007 |
| Last update date |
Apr 09, 2008 |
| Contact name |
Guoying Wang |
| E-mail(s) |
gywang@caas.net.cn
|
| Phone |
86-10-62162927
|
| Organization name |
Chinese Academy of Agricultural Sciences
|
| Department |
Institute of Crop Sciences
|
| Lab |
Maize Molecular Biology
|
| Street address |
Zhongguancun Nandajie 12
|
| City |
Beijing |
| ZIP/Postal code |
100081 |
| Country |
China |
| |
|
| Platform ID |
GPL5440 |
| Series (1) |
| GSE9386 |
Genome-wide analysis of gene expression profiles during the kernel development of maize (Zea mays L.) |
|
