|
| Status |
Public on Nov 20, 2017 |
| Title |
db/db HSC, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Hematopoietic stem cells from db/db mice
|
| Organism |
Mus musculus |
| Characteristics |
cell type: bone marrow hematopoietic stem cells
genotype: leptin receptor mutation
age: 3 month old mice
|
| Growth protocol |
hematopoietic stem cells were sorted by flowcytometry
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA were isolated by mirVana miRNA isolation kit according to manufacturer's instructions (Applied Biosystems).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ng total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation, 20 ng of total RNA were hybridized for 16 hr at 45C. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned by the Affymetrix Scanner 3000(Affymetrix).
|
| Description |
microRNA expression in hematopoietic stem cells
|
| Data processing |
The scanned images were analyzed using Expression Console Software (version 1.3.1 Affymetrix)
affymetrix-algorithm-name = rma : dabg
|
| |
|
| Submission date |
Nov 14, 2016 |
| Last update date |
Nov 20, 2017 |
| Contact name |
Lyne Khair |
| Organization name |
UMASS MEDICAL SCHOOL
|
| Department |
SURGERY
|
| Street address |
368 PLANTATION STREET
|
| City |
WORCESTER |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE89842 |
Diabetes Impairs Wound-Healing by let-7d-3p-dependent DNMT1 Upregulation in Hematopoietic Stem Cells |
|
