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Sample GSM239170 Query DataSets for GSM239170
Status Public on Nov 01, 2007
Title BM_CD34_R000030
Sample type RNA
 
Source name Bone Marrow CD34+ selected cells
Organism Homo sapiens
Characteristics Bone Marrow CD34+ selected cells
Unknown age/Male
Extracted molecule total RNA
Extraction protocol Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
Label Biotin
Label protocol Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life TEchnologies, Farmingdale, NY) according to manufacturer's protocol.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocol.
Scan protocol Arrays were scanned according to Affymetrix protocol.
Description n/a
Data processing .DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Log2 expression values for individual probe sets were generated from .CEL files via robust multi-array average (gcRMA).
 
Submission date Oct 22, 2007
Last update date Nov 14, 2018
Contact name Derek L Stirewalt
E-mail(s) dstirewa@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Clinical Research Division
Lab Stirewalt Lab
Street address 1100 Fairview Ave N, D5-112
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL96
Series (1)
GSE9476 Abnormal Expression Changes in AML
Relations
Reanalyzed by GSM339908
Reanalyzed by GSM915756
Reanalyzed by GSE122505

Data table header descriptions
ID_REF
VALUE Log2 Expression value obtained from gcRMA analyses of .CEL file

Data table
ID_REF VALUE
1007_s_at 3.016704177
1053_at 7.977735461
117_at 4.207280707
121_at 7.25609471
1255_g_at 2.204955099
1294_at 7.28437278
1316_at 4.265792764
1320_at 2.6943516
1405_i_at 3.646303109
1431_at 3.040292001
1438_at 2.357625231
1487_at 5.378494673
1494_f_at 2.833814735
1598_g_at 6.192932281
160020_at 4.543042482
1729_at 6.069437462
177_at 3.241442467
1773_at 2.223038163
179_at 6.839150887
1861_at 1.770783478

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM239170.CEL.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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