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Sample GSM239490 Query DataSets for GSM239490
Status Public on Nov 01, 2007
Title FHCRCAML_2762
Sample type RNA
 
Source name Leukemia
Organism Homo sapiens
Characteristics Diagnosis: MDS/AML
Age: 79y
Gender: Male
Blast %: 87
Source: PB
Cyto: Normal
FLT3 ITD: 0
FLT3 TKD: 0
Extracted molecule total RNA
Extraction protocol Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
Label Biotin
Label protocol Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life TEchnologies, Farmingdale, NY) according to manufacturer's protocol.
 
Hybridization protocol Hybridization was performed according to Affymetrix protocol.
Scan protocol Arrays were scanned according to Affymetrix protocol.
Description n/a
Data processing .DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Log2 expression values for individual probe sets were generated from .CEL files via robust multi-array average (gcRMA).
 
Submission date Oct 24, 2007
Last update date Nov 14, 2018
Contact name Derek L Stirewalt
E-mail(s) dstirewa@fhcrc.org
Organization name Fred Hutchinson Cancer Research Center
Department Clinical Research Division
Lab Stirewalt Lab
Street address 1100 Fairview Ave N, D5-112
City Seattle
State/province WA
ZIP/Postal code 98109
Country USA
 
Platform ID GPL96
Series (1)
GSE9476 Abnormal Expression Changes in AML
Relations
Reanalyzed by GSM915785
Reanalyzed by GSE122505

Data table header descriptions
ID_REF
VALUE Log2 Expression value obtained from gcRMA analyses of .CEL file

Data table
ID_REF VALUE
1007_s_at 2.994833898
1053_at 6.812653457
117_at 4.027821536
121_at 7.094220515
1255_g_at 2.191163224
1294_at 9.531410347
1316_at 4.462643546
1320_at 2.64768862
1405_i_at 7.339138016
1431_at 3.038659892
1438_at 2.307429686
1487_at 5.494600863
1494_f_at 2.802912751
1598_g_at 5.965318286
160020_at 4.528950974
1729_at 6.355952696
177_at 4.084397245
1773_at 2.222344633
179_at 6.820596415
1861_at 1.970035467

Total number of rows: 22283

Table truncated, full table size 496 Kbytes.




Supplementary file Size Download File type/resource
GSM239490.CEL.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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