|
| Status |
Public on Nov 01, 2007 |
| Title |
FHCRCAML3653 |
| Sample type |
RNA |
| |
|
| Source name |
Leukemia
|
| Organism |
Homo sapiens |
| Characteristics |
Diagnosis: AML M1
Age: 52y
Gender: Female
Blast %: 88
Source: BM
Cyto: Complex
FLT3 ITD: 0
FLT3 TKD: 0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using TRIzol reagent (Invitrogen, Carlsbad, CA)according to manufacturer's protocol.
|
| Label |
Biotin
|
| Label protocol |
Target was labeled with Enzo BioArray High Yield RNA Trascript Labeling Kit (Enzo Life TEchnologies, Farmingdale, NY) according to manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed according to Affymetrix protocol.
|
| Scan protocol |
Arrays were scanned according to Affymetrix protocol.
|
| Description |
n/a
|
| Data processing |
.DAT files were generated using GCOS 1.2.1 software (Affymetrix). CEL files were generated using MAS 5.0 softare (Affymetrix) with target signals for probe sets scaled to 500. Log2 expression values for individual probe sets were generated from .CEL files via robust multi-array average (gcRMA).
|
| |
|
| Submission date |
Oct 24, 2007 |
| Last update date |
Nov 14, 2018 |
| Contact name |
Derek L Stirewalt |
| E-mail(s) |
dstirewa@fhcrc.org
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Clinical Research Division
|
| Lab |
Stirewalt Lab
|
| Street address |
1100 Fairview Ave N, D5-112
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE9476 |
Abnormal Expression Changes in AML |
|
| Relations |
| Reanalyzed by |
GSM915789 |
| Reanalyzed by |
GSE122505 |
