|
| Status |
Public on Oct 26, 2007 |
| Title |
spt3_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
spt3 strain
|
| Organism |
Saccharomyces cerevisiae |
| Characteristics |
MATα ura3-52 trp1 leu2Δ::PET56 gcn4 gal2 spt3::TRP1
|
| Growth protocol |
Cells were grown for 24 hr at 30°C in liquid glucose-rich medium and then transferred at an OD600 of 0.2 to 37.5°C (restrictive temperature). RNA was extracted when the cells were at an OD600 of 0.8.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
acid phenol method
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedure
|
| |
|
| Hybridization protocol |
standard Affymetrix procedure
|
| Scan protocol |
scan analyzed by Affymetrix' MicroArraySuite, MicroDB and DataMiningTool
|
| Description |
na
|
| Data processing |
Signal intensities from CHP using ArrayAssist v2.6. Significance level set to P=0.05. Only mRNAs expressed at similar levels in the duplicate samples which were also signicantly different in the other strains were analyzed.
|
| |
|
| Submission date |
Oct 25, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Martine Collart |
| E-mail(s) |
martine.collart@unige.ch
|
| Organization name |
University of Geneva
|
| Department |
Microbiology and Molecular Medicine
|
| Street address |
Rue Michel-Servet 1, Centre Médical Universitaire
|
| City |
Geneva |
| ZIP/Postal code |
1206 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL90 |
| Series (1) |
| GSE9432 |
A SAGA-Independent Function of SPT3 Mediates Transcriptional Deregulation in a Mutant of the Ccr4-Not Complex |
|
