|
| Status |
Public on Nov 24, 2016 |
| Title |
HC_04 |
| Sample type |
SRA |
| |
|
| Source name |
Whole blood cells (PAX)
|
| Organism |
Homo sapiens |
| Characteristics |
clinical status: Healthy Control
dmards treatment: NO
group: No RA
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted using PAXgene Blood miRNA kit (QIAGEN) following the manufacturer protocol
RNA libraries were prepared using standard illumina TruSeq RNA protocol, following PolyA enrichment
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Data processing |
Mapping to human referense assembly hg19 was performed with Tophat/1.3.3
Duplicate removal on mapped data was performed with picard-tools/1.29
FPKM values were calculated with cufflinks/1.3.0
The above analysies were performed in complience with the protocol of Science for Life Laboratory, Stockholm, Sweden
hg19
Supplementary_files_format_and_content: Tab-delimited table with merged FPKM values for each sample
|
| |
|
| Submission date |
Nov 18, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Klementy Shchetynsky |
| E-mail(s) |
klementy.shchetynsky@ki.se
|
| Organization name |
Karolinska Institutet
|
| Department |
Medicine, Solna
|
| Lab |
Rheumatology Research
|
| Street address |
CMM L8:04 Karolinska University Hospital Solna
|
| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE90081 |
Discovery of new candidate genes for rheumatoid arthritis through integration of genetic association data with expression pathway analysis |
|
| Relations |
| BioSample |
SAMN06041115 |
| SRA |
SRX2362416 |
