|
Status |
Public on Nov 24, 2016 |
Title |
HC_11 |
Sample type |
SRA |
|
|
Source name |
Whole blood cells (PAX)
|
Organism |
Homo sapiens |
Characteristics |
clinical status: Healthy Control dmards treatment: NO group: No RA
|
Extracted molecule |
polyA RNA |
Extraction protocol |
RNA was extracted using PAXgene Blood miRNA kit (QIAGEN) following the manufacturer protocol RNA libraries were prepared using standard illumina TruSeq RNA protocol, following PolyA enrichment
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Mapping to human referense assembly hg19 was performed with Tophat/1.3.3 Duplicate removal on mapped data was performed with picard-tools/1.29 FPKM values were calculated with cufflinks/1.3.0 The above analysies were performed in complience with the protocol of Science for Life Laboratory, Stockholm, Sweden hg19 Supplementary_files_format_and_content: Tab-delimited table with merged FPKM values for each sample
|
|
|
Submission date |
Nov 18, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Klementy Shchetynsky |
E-mail(s) |
klementy.shchetynsky@ki.se
|
Organization name |
Karolinska Institutet
|
Department |
Medicine, Solna
|
Lab |
Rheumatology Research
|
Street address |
CMM L8:04 Karolinska University Hospital Solna
|
City |
Stockholm |
State/province |
Stockholm |
ZIP/Postal code |
171 76 |
Country |
Sweden |
|
|
Platform ID |
GPL11154 |
Series (1) |
GSE90081 |
Discovery of new candidate genes for rheumatoid arthritis through integration of genetic association data with expression pathway analysis |
|
Relations |
BioSample |
SAMN06041109 |
SRA |
SRX2362423 |