|
Status |
Public on Nov 20, 2007 |
Title |
HGF + UO, biological rep 1 |
Sample type |
RNA |
|
|
Source name |
Madin Darby canine kidney (MDCK) cells, exposed to HGF + UO
|
Organism |
Canis lupus familiaris |
Characteristics |
MDCK type II cells
|
Treatment protocol |
MDCK cells grown on a Transwell filter for 7 days exposed to 24 hours of hepatocyte growth factor at 100 ng/ml and UO126 at a concentration of 10 uM
|
Growth protocol |
MDCK cells grown on a Transwell filter in a 37 °C 5% CO2 incubator and fed MEM + 5% FBS
|
Extracted molecule |
total RNA |
Extraction protocol |
Qiagen extraction of total RNA according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microgram total RNA (Expression Analysis Technical Manual, 2001, Affymetrix)
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|
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Hybridization protocol |
Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on Canine Genome 2.0 Array (Affymetrix).
|
Scan protocol |
GeneChips were scanned at 3-nm resolution after excitation at 570 nm.
|
Description |
Gene expression data from MDCK cells grown on a Transwell filter and exposed to HGF + UO
|
Data processing |
GCRMA-calculated Signal Intensity and Affymetrix flags using ArrayAssist Lite v3.4, Stratagene
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|
|
Submission date |
Oct 26, 2007 |
Last update date |
Aug 14, 2011 |
Contact name |
Xiaofeng Zuo |
Organization name |
University of Pennsylvania
|
Lab |
Josh Lipschutz
|
Street address |
380 S. University Ave.
|
City |
philadelphia |
State/province |
PA |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL3738 |
Series (1) |
GSE9435 |
Expression data from MDCK cells exposed to HGF +/- MAPK inhibitors |
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