|
| Status |
Public on Sep 15, 2017 |
| Title |
Normal Human Astrocytes |
| Sample type |
RNA |
| |
|
| Source name |
Normal Human Astrocytesgrown under standard methods
|
| Organism |
Homo sapiens |
| Characteristics |
location: not available
gender: not available
sample type: normal human astrocyte cell line
|
| Treatment protocol |
Not Applicable
|
| Growth protocol |
Normal Human Astrocytes were obtained from Lonza ( NJ, USA) and grown with AGM BulletKit (Lonza, NJ, USA). Human Fetal Astrocytes were obtained from ABM (Richmond, Canada), grown in collagen coated plates according to manufacturer’s protocol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Fragmented and Labeled ss-cDNA were prepared according to WT PLUS Reagent Kit from 500 ng total RNA
|
| |
|
| Hybridization protocol |
943 ng of ss-cDNA were hybridized for 20 hr at 48C on Human Gene 2.1 ST Array. Array strpis were washed and stained in the GeneAtlas Fluidics station (Affymetrix)
|
| Scan protocol |
GeneChips were scanned using the GeneAtlas Array scanner
|
| Description |
miRNA expression data from normal human astrocyte cell line
|
| Data processing |
The resulting data were analyzed with Transcriptome Analysis Console 3 software and DAVID online tool
|
| |
|
| Submission date |
Nov 28, 2016 |
| Last update date |
Sep 15, 2017 |
| Contact name |
Sukru Gulluoglu |
| E-mail(s) |
sgulluoglu@gmail.com
|
| Organization name |
Yeditepe University
|
| Street address |
Acibadem Mah. IStek vakfi binasi
|
| City |
IStanbul |
| ZIP/Postal code |
34587 |
| Country |
Turkey |
| |
|
| Platform ID |
GPL21572 |
| Series (2) |
| GSE90603 |
miRNA Expression data from Glioblastoma Multiforme patient tumor samples, healthy brain tissue (partly from GBM patients) and NHA cell line and Human fetal astrocyte cell line |
| GSE90604 |
Expression data from Glioblastoma Multiforme patient tumor samples, healthy brain tissue (partly from GBM patients) and NHA cell line and Human fetal astrocyte cell line |
|
