|
| Status |
Public on Nov 30, 2007 |
| Title |
rat kidney at week 4 post birth, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
rat kidney at week 4 post birth, rep3
|
| Organism |
Rattus norvegicus |
| Characteristics |
rat kidney at week 4 post birth, rep3
|
| Growth protocol |
Embryonic kidneys were immediately processed after dissection. Recombination was cultured for 6 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using the Ambion Acqueous micro kit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat 230 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
none
|
| Data processing |
The data were analyzed with GCOS using Affymetrix default analysis settings
|
| |
|
| Submission date |
Nov 08, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Sanjay Nigam |
| Organization name |
University of California, San Diego
|
| Department |
Pediatrics
|
| Lab |
Nigam Lab
|
| Street address |
9500 Gilman Dr
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093-0693 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE9570 |
Expression data from embryonic rat kidney timepoints and a budded WD-MM recombination tissue |
|
