|
| Status |
Public on May 03, 2017 |
| Title |
KSHV-VLV (microRNA) |
| Sample type |
SRA |
| |
|
| Source name |
KSHV-65stop
|
| Organisms |
Homo sapiens; Human gammaherpesvirus 8 |
| Characteristics |
cell type: iSLK (epithelial cell)
virus: KSHV-65stop
|
| Treatment protocol |
iSLK-KSHV-65stop cells were treat with TPA +NaB for three day, and then KSHV VLVs were purified from supernatant through sucrose density gradient
|
| Growth protocol |
iSLK cells latently infected with KSHV-65stop
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol for total RNA, and then gel purify microRNA
TruSeq Small RNA Library Prep Kit
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
no
|
| Data processing |
TopHat under default parameter
Genome_build: hg38, KSHV JSC-1 clone BAC16
Supplementary_files_format_and_content: counts
|
| |
|
| Submission date |
Dec 09, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Danyang Gong |
| E-mail(s) |
gongdanyang@gmail.com
|
| Phone |
3107945125
|
| Organization name |
UCLA
|
| Department |
pharmacology
|
| Lab |
Ren Sun lab
|
| Street address |
650 Charles Young Dr. So.
|
| City |
los angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
|
| Platform ID |
GPL19367 |
| Series (2) |
| GSE91385 |
Virus-like vesicles of Kaposi’s Sarcoma-Associated Herpesvirus activate lytic replication through triggering differentiation signaling (microRNA) |
| GSE91386 |
Virus-like vesicles of Kaposi’s Sarcoma-Associated Herpesvirus activate lytic replication through triggering differentiation signaling |
|
| Relations |
| BioSample |
SAMN06128299 |
| SRA |
SRX2409669 |
