|
| Status |
Public on Dec 13, 2016 |
| Title |
male-small intestine-sex-matched-rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
male, small intestine, sex-matched FMT, 6.5 Gy， rep 3
|
| Organism |
Mus musculus |
| Characteristics |
tissue: small intestine
gender: male
|
| Treatment protocol |
Conventional, untreated, age-matched male and female were used as donors to collect gut microbiota. The donor’s fecal droppings were collected under SPF conditions. Donor stool was freshly prepared on the day of transplant and that in all cases was prepared and transplanted within 4 h. Donor stool was weighed and diluted with 1 mL of saline per 0.1 g of stool.
|
| Growth protocol |
Mice were kept under standard conditions (ambient temperature 22±2℃, air humidity 40–70% and a 12/12-h light/dark cycle) and continuous access to a standard diet and water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using the TRIzol reagent following the manufacturer's recommendations. RNA was quantified using a NanoDrop-1000 spectrophotometer.
|
| Label |
Cy3
|
| Label protocol |
cDNA labeled with a fluorescent dye (Cy3-dCTP) was produced by Eberwine’s linear RNA amplification method and subsequent enzymatic reaction. This procedure has been previously described, and the procedure has been improved by using CapitalBio cRNA Amplification and Labeling Kit (CapitalBio) for producing higher yields of labeled cDNA.
|
| |
|
| Hybridization protocol |
DNA in hybridization solution was denatured at 95℃ for 3 min prior to loading onto a microarray. Arrays were hybridized was preformed in a Agilent Hybridization Oven overnight at a rotation speed of 20 rpm at 42℃ and washed with two consecutive solutions (0.2% SDS, 2× SSC at 42℃ for 5 min, and 0.2× SSC for 5 min at room temperature).
|
| Scan protocol |
Scanned on an Agilent G2565CA scanner.
|
| Description |
Gene expression after 6.5 Gy irradiation with treatment of sex-matched FMT
|
| Data processing |
Feature Extraction v10.7 (Agilent Technologies, CA) software was used to extract all features of the data obtained from the scanned images and genespring software was used to analyze the raw data, which are normalized by percentile normalization.
|
| |
|
| Submission date |
Dec 12, 2016 |
| Last update date |
Dec 13, 2016 |
| Contact name |
Ming Cui |
| E-mail(s) |
cuiming0403@bjmu.edu.cn
|
| Organization name |
Chinese Academy of Medical Sciences and Peking Union Medical College
|
| Department |
Institute of Radiation Medicine,
|
| Street address |
238 Baidi Road
|
| City |
Tianjin |
| ZIP/Postal code |
300192 |
| Country |
China |
| |
|
| Platform ID |
GPL22782 |
| Series (1) |
| GSE92249 |
Gene expression profile of small intestine tissue from C57BL/6 mice with or without faecal microbiota transplantation |
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