|
| Status |
Public on Dec 13, 2016 |
| Title |
4T1-HAS1DN in RAGKO + WT CTL |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
HA/dominant-negaive form of STAT1-transfected 4T1 tumor cells after the growth in RAG-/- treated with WT CTL
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 4T1
tissue: mammary tumor cell line
strain: BALB/c
|
| Treatment protocol |
Tumor cells were isolated from the out-growing tumor mass in the indicated mice and the primarily inoculated tumor cells was used as reference. Some mice were treated with IFN-g-/-, perforin-/- or WT CTL or IL-12. 4T1-HA cells cultured with IFN-g were also examined and the tumor cells at the starting point of culture was used as reference.
|
| Growth protocol |
4T1-HAS1DN cells kept in complete RPMI1640-10% FCS medium after the establishment by dominant-negative form of STAT1 gene transfection into HA-expressing 4T1 (4T1-HA) tumor cells follwed by single cell cloning.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using DNeasy Blood & Tissue Kit (QUIAGEN) following manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
500ng DNA was labelled using the SureTag DNA Labeling Kit coupling method (Agilent – 5190-3400).
|
| |
|
| Channel 2 |
| Source name |
HA/dominant-negaive form of STAT1-transfected 4T1 tumor cells that was primarily inoculated into the mice
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 4T1
tissue: mammary tumor cell line
strain: BALB/c
|
| Treatment protocol |
Tumor cells were isolated from the out-growing tumor mass in the indicated mice and the primarily inoculated tumor cells was used as reference. Some mice were treated with IFN-g-/-, perforin-/- or WT CTL or IL-12. 4T1-HA cells cultured with IFN-g were also examined and the tumor cells at the starting point of culture was used as reference.
|
| Growth protocol |
4T1-HAS1DN cells kept in complete RPMI1640-10% FCS medium after the establishment by dominant-negative form of STAT1 gene transfection into HA-expressing 4T1 (4T1-HA) tumor cells follwed by single cell cloning.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA extracted using DNeasy Blood & Tissue Kit (QUIAGEN) following manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
500ng DNA was labelled using the SureTag DNA Labeling Kit coupling method (Agilent – 5190-3400).
|
| |
|
| |
| Hybridization protocol |
Labelled samples were hybridized on SurePrint G3 Mouse CGH Microarray 4x180K (Agilent) according to the manufacturer's instructions.
|
| Scan protocol |
Microarray slides were scanned in an Agilent Technologies G2505C Microarray Scanner at 3 micron resolution.
|
| Description |
4T1-HAS1N cells grown in RAG -/- mice treated with WT CTL
|
| Data processing |
Images were analyzed with Feature Extraction Software 10.7.3.1 (Agilent Technologies), in accordance to the CGH_107_Sep09 protocol for background subtraction and normalization.
|
| |
|
| Submission date |
Dec 12, 2016 |
| Last update date |
Dec 13, 2016 |
| Contact name |
Kazuyoshi Takeda |
| E-mail(s) |
ktakeda@juntendo.ac.jp
|
| Phone |
81-3-5802-1045
|
| Organization name |
Juntendo Univ.
|
| Department |
Biomedical Research Center
|
| Lab |
Division of Cell Biology
|
| Street address |
2-1-1 Hongo
|
| City |
Bunkyo-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
113-8421 |
| Country |
Japan |
| |
|
| Platform ID |
GPL10449 |
| Series (2) |
| GSE92266 |
Copy number alteration in Murine Tumor Cells: primary inoculated tumor cells vs. out-growing tumor cells [Fig3b] |
| GSE92271 |
Copy number alteration in Murine Tumor Cells: primary inoculated tumor cells vs. out-growing tumor cells |
|
