|
| Status |
Public on Dec 13, 2016 |
| Title |
Drosophila melanogaster Tailor mutant testis total RNA replicate 2 |
| Sample type |
SRA |
| |
|
| Source name |
dissected testes
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: testes
genotype: tailor mutant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were isolated from w1118, Tailor, Dis3L2, and Tailor/Dis3L2 mutant 5 day old fly testes using Trizol. Two independent dissections were made to generate biologically replicate RNA samples, whose quality was assessed by Bioanalyzer
The Illumina Truseq Total RNA library Prep Kit LT was used to make RNAseq libraries from 650 ng of total RNA. Manufacturer’s protocol was followed except for using 8 cycles of PCR to amplify the final library instead of the recommended 15 cycles, to minimize artifacts caused by PCR amplification. All samples were pooled together, using the barcoded adapters provided by the manufacturer, over 2 flow cells of a HiSeq2500 and sequenced using pair-end PE50 at the New York Genome Center.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sample name: G0033
|
| Data processing |
3' adapter sequences were trimmmed using Trimmomatic-0.36
Total RNA-seq paired-end reads from four datasets to UCSC Drosophila melanogaster (dm6) genome assembly using HISAT2
Genome_build: dm6
Supplementary_files_format_and_content: raw counts of genes
|
| |
|
| Submission date |
Dec 12, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jiayu Wen |
| Organization name |
John Curtin School of Medical Research, The Australian National University
|
| Department |
Department of Genome Science
|
| Lab |
The Wen Lab
|
| Street address |
133 Garran Rd
|
| City |
CANBERRA |
| State/province |
ACT |
| ZIP/Postal code |
2601 |
| Country |
Australia |
| |
|
| Platform ID |
GPL17275 |
| Series (1) |
| GSE92305 |
Characterization of a TUTase/nuclease complex required for Drosophila gametogenesis |
|
| Relations |
| Reanalyzed by |
GSM3282543 |
| BioSample |
SAMN06134392 |
| SRA |
SRX2416973 |
