|
| Status |
Public on Mar 09, 2017 |
| Title |
HUVEC GATA4 biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
HUVEC transduced with Gata4 vector
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HUVEC
transduction: GATA4
|
| Treatment protocol |
Filtered supernatants from Phoenix cells containing retroviral particles were applied to HUVEC at three consecutive days. Before harvesting the cells (day 7) puromycin (Invitrogen) was added (day 5) at a concentration of 1 μg/ml to GATA4- or empty vector-transduced HUVEC.
|
| Growth protocol |
Human GATA4 cDNA (IMAGE:8144111) was cloned into pBabePuro vector (Addgene). To obtain recombinant retroviral particles, Phoenix producer cells [ATCC CRL-3213] were lipofected with vector DNA as described by Viemann D et al, 2007.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total was RNA from GATA4- or empty vector-transduced HUVEC was extracted with EZNA TM Total-RNA-Kit according to the manufacturer's protocol (OMEGA Biotec).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Gene expression data from GATA4-transduced HUVEC
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 6, from SAS. Gene expression profiling was performed using arrays of human HG-U133_Plus_2 type from Affymetrix. A Custom CDF Version 14 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary
|
| |
|
| Submission date |
Dec 13, 2016 |
| Last update date |
Mar 09, 2017 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL16311 |
| Series (2) |
| GSE92354 |
GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis (2 of 3) |
| GSE92357 |
GATA4-dependent organ-specific endothelial differentiation controls liver development and embryonic hematopoiesis |
|
