|
| Status |
Public on Dec 15, 2016 |
| Title |
S1_Lhx2_WT_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
WT S1 cortex
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
age: P7
tissue: primary somatosensory cortex
|
| Treatment protocol |
Primary somatosensory cortices were dissected from P7 mice brains.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TriPure Isolation Reagent (Roche) following the manufacture's insreuctions. RNA was quantified using a NanoDrop-1000 spectrophotometer and quality was monitored with the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
|
| Label |
Cy3
|
| Label protocol |
0.2 μg of total RNA was amplified by a Low Input Quick-Amp Labeling kit (Agilent Technologies, USA) and labeled with Cy3 (CyDye, Agilent Technologies, USA) during the in vitro transcription process.
|
| |
|
| Hybridization protocol |
0.6 μg of Cy3-labled cRNA was fragmented to an average size of about 50-100 nucleotides by incubation with fragmentation buffer at 60°C for 30 minutes. Correspondingly fragmented labeled cRNA is then pooled and hybridized to Agilent SurePrint G3 Mouse GE 8×60K Microarray (Agilent Technologies, USA) at 65°C for 17 h.
|
| Scan protocol |
After washing and drying by nitrogen gun blowing, microarrays were scanned with an Agilent microarray scanner (Agilent Technologies, USA) at 535 nm for Cy3.
|
| Description |
Gene expression in wild-type S1
|
| Data processing |
Scanned images were analyzed by Feature extraction10.5.1.1 software (Agilent Technologies, USA), an image analysis and normalization software was used to quantify signal and background intensity for each feature.
|
| |
|
| Submission date |
Dec 14, 2016 |
| Last update date |
Dec 15, 2016 |
| Contact name |
Shen-Ju Chou |
| Organization name |
Academia Sinica
|
| Department |
Institute of Cellular and Organismic Biology
|
| Street address |
128 Sec. 2, Academia Rd, Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL10787 |
| Series (1) |
| GSE92372 |
Expression profile of P7 control and Lhx2 cKO somatosensory cortex |
|
