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Sample GSM242975 Query DataSets for GSM242975
Status Public on Dec 09, 2008
Title Ferret lung with H3N2 at Day 6, biological rep3
Sample type RNA
 
Source name Ferret
Organism Mustela putorius furo
Characteristics Tissue: lung
Virus Strain: H3N2
Treatment protocol Ferrets were inoculated intranasally with 10(6) EID50 of either A/Vietnam/1203/04 (H5N1) or A/Panama/2007/99 (H3N2). At 2, 4 and 6 days post-infection (DPI), ferrets were euthanized and lung tissue was excised and placed on ice in the TRIzol solution (Invitrogen) and homogenized immediately.
Extracted molecule total RNA
Extraction protocol TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Description Gene expression data from ferret on Day 6 after infection
Data processing The data were analyzed with PLIER using ArrayAssist (Stratagene) default analysis settings.
 
Submission date Nov 14, 2007
Last update date Aug 14, 2011
Contact name Longsi Ran
E-mail(s) lran@uhnresearch.ca
Phone 416-581-7607
Organization name Toronto General Research Institute
Department Division of Experimental Therapeutics
Lab Dr. David J Kelvin
Street address 101 College Street
City Toronto
State/province ON
ZIP/Postal code M5G 1L7
Country Canada
 
Platform ID GPL3738
Series (1)
GSE9606 Lungs of ferrets infected with either an H3N2 or H5N1 subtype of influenza A virus.

Data table header descriptions
ID_REF
VALUE PLIER-calculated Signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1630.7268
AFFX-BioB-M_at 2126.5564
AFFX-BioB-3_at 1417.5037
AFFX-BioC-5_at 3579.0884
AFFX-BioC-3_at 3718.336
AFFX-BioDn-5_at 6087.7954
AFFX-BioDn-3_at 6558.356
AFFX-CreX-5_at 13569.114
AFFX-CreX-3_at 11390.599
AFFX-DapX-5_at 1653.8522
AFFX-DapX-M_at 8153.568
AFFX-DapX-3_at 9902.668
AFFX-LysX-5_at 451.0611
AFFX-LysX-M_at 2432.8328
AFFX-LysX-3_at 6888.153
AFFX-PheX-5_at 1181.4688
AFFX-PheX-M_at 3732.5688
AFFX-PheX-3_at 5213.1978
AFFX-ThrX-5_at 872.81433
AFFX-ThrX-M_at 4692.0938

Total number of rows: 43035

Table truncated, full table size 1263 Kbytes.




Supplementary file Size Download File type/resource
GSM242975.CEL.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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