|
| Status |
Public on Dec 09, 2008 |
| Title |
Ferret lung with H5N1 at Day 4, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Ferret
|
| Organism |
Mustela putorius furo |
| Characteristics |
Tissue: lung
Virus Strain: H5N1
|
| Treatment protocol |
Ferrets were inoculated intranasally with 10(6) EID50 of either A/Vietnam/1203/04 (H5N1) or A/Panama/2007/99 (H3N2). At 2, 4 and 6 days post-infection (DPI), ferrets were euthanized and lung tissue was excised and placed on ice in the TRIzol solution (Invitrogen) and homogenized immediately.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix Two-Cycle protocol from 100 ng total RNA (Expression Analysis Technical Manual, 2004, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microgram of cRNA were hybridized for 16 hr at 45C on GeneChip Canine Genome 2.0 Array in Affymetrix Hybridization Oven 640. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from ferret on Day 4 after infection
|
| Data processing |
The data were analyzed with PLIER using ArrayAssist (Stratagene) default analysis settings.
|
| |
|
| Submission date |
Nov 14, 2007 |
| Last update date |
Aug 14, 2011 |
| Contact name |
Longsi Ran |
| E-mail(s) |
lran@uhnresearch.ca
|
| Phone |
416-581-7607
|
| Organization name |
Toronto General Research Institute
|
| Department |
Division of Experimental Therapeutics
|
| Lab |
Dr. David J Kelvin
|
| Street address |
101 College Street
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7 |
| Country |
Canada |
| |
|
| Platform ID |
GPL3738 |
| Series (1) |
| GSE9606 |
Lungs of ferrets infected with either an H3N2 or H5N1 subtype of influenza A virus. |
|
