Biotinylated cRNA were prepared according to the standard affymetrix protocol
Hybridization protocol
Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol
GeneChips were scanned using GeneChip Scanner 3000
Description
human peripheral blood B cells B24 WM B cells_GPL91
Data processing
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
