|
| Status |
Public on Dec 26, 2018 |
| Title |
mouse spleen (+tumor cells injected) at T1 |
| Sample type |
RNA |
| |
|
| Source name |
C57BL/6N mice (B6, Shizuoka, Japan) and C57BL/6-Il10tm1Cgn mice
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6N and C57BL/6-Il10tm1Cgn
tissue: spleen
cell markers: CD11b+Gr1hi
age: 8-12-week
gender: female
|
| Treatment protocol |
LPS i.p. ( L, LH group) at "0", HY209 i.v (LH group) at 30 min after LPS injection, EL4 tumor cells s.c. (T group)
|
| Growth protocol |
CD11b+Gr1hi cells were FACS-purified from the spleen of mice at 24h after LPS injection (L1 and L2), HY209 was infused intravenously into the group of mice at 30 min after LPS injection (LH1, LH2). CD11b+Gr1hi cells were FACS-purified from the spleen of EL4 tumor-bearing mice (T1, T2)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip 3' IVT PLUS Reagent Kit Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45℃ on GeneChip Hybridization Oven 645. GeneChips were washed and stained with SAPE (Streptavidin Phycoerythrin) in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
Sample name: T1
Gene expression data from mouse spleen
|
| Data processing |
The data were analyzed with AffymetrixExpressionConsole_1_4_1 (MAS5)
|
| |
|
| Submission date |
Dec 27, 2016 |
| Last update date |
Dec 26, 2018 |
| Contact name |
Seung-yong Seong |
| E-mail(s) |
seongsy@snu.ac.kr
|
| Phone |
+82-2-740-8301
|
| Organization name |
Wide River Institute of Immunology, Seoul National University College of Medicine
|
| Department |
Microbiology and Immunology
|
| Lab |
Hyppo Lab.
|
| Street address |
28 Yongon-dong, Jongno-gu
|
| City |
Seoul |
| ZIP/Postal code |
110-799 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE92948 |
GPCR19 agonist increases in number of immune-regulatory myeloid cells and their expression of immune checkpoint molecule |
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