|
| Status |
Public on Aug 28, 2017 |
| Title |
6hrwounding_CtrlEpdSC__R2_RNA-seq |
| Sample type |
SRA |
| |
|
| Source name |
Skin
|
| Organism |
Mus musculus |
| Characteristics |
Sex: female
strain: C57BL/6
cell type: adult skin epithelia
sort markers: Integrin A6+, B1+ SCa1+ CD34-
treatment: vanicream for 6 days and wounded at 30 days. 6hr wounds were harvested
|
| Treatment protocol |
postnatal day 60 mice were treated with vanicream for 6 days and wounded at 30 days. 6hr wounds were harvested
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were FACS sorted according to characteristics: sort markers directly into Trizol. Total RNA extracted via Trizol/Zymo RNA extraction kit.
Illumina TruSeq RNA Sample Preparation
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
Sample 10
mRNA library made from total RNA
|
| Data processing |
50-bp sequences were aligned to mm10 using STAR and Gencode release M9 and counts generated from STAR output --quantMode GeneCounts.
Genome_build: mm10
Supplementary_files_format_and_content: GENE_ID, Counts
|
| |
|
| Submission date |
Dec 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Nicholas Gomez |
| Organization name |
Rockefeller University
|
| Street address |
1230 York Ave
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE92967 |
Inflammatory Memory in Epidermal Stem Cells Accelerates Tissue Damage Responses |
|
| Relations |
| BioSample |
SAMN06187818 |
| SRA |
SRX2448165 |
