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Status
Public on Sep 19, 2002
Title
B7hi mTEC B6/129 wt2
Sample type
RNA
Source name
murine medullary thymic epithelial cells
Organism
Mus musculus Extracted molecule
total RNA
Description
Mice used were wild type B6/129 F2's of 3-5 weeks of age. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Submission date
Sep 18, 2002
Last update date
May 27, 2005
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL81 Series (1)
GSE85 wild type and aire -/- murine meduallary thymic epithelial cells
Data table header descriptions ID_REF VALUE Microarray Suite 5.0 raw signal
ABS_CALL Microarray Suite 5.0 call -- absent, present, marginal
NORM_VALUE signal values after processing with "Norm" software
Data table ID_REF VALUE ABS_CALL NORM_VALUE 100001_at
1.3
A
1.19
100002_at
12.1
A
11.39
100003_at
2.8
A
2.54
100004_at
9
P
8.51
100005_at
9.9
A
9.45
100006_at
6.3
M
5.85
100007_at
11.2
A
10.64
100009_r_at
31.4
P
30.7
100010_at
1
A
1
100011_at
53.5
P
53.8
100012_at
488
P
569.18
100013_at
28.6
P
31.85
100014_at
5.6
P
5.28
100015_at
15.1
P
15.06
100016_at
7.9
A
7.37
100017_at
7.7
A
7.21
100018_at
31.5
A
30.04
100019_at
8.4
P
7.85
100020_at
28.2
P
28.7
100021_at
7.1
A
6.51
12488 260 Kbytes .Supplementary file Size Download File type/resource GSM2445.CEL.gz
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