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Sample GSM244768 Query DataSets for GSM244768
Status Public on Jan 01, 2008
Title Treated CD colon repl5
Sample type RNA
 
Source name human colon
Organism Homo sapiens
Characteristics treated CD replicate sample
batch 4
Extracted molecule total RNA
Extraction protocol RNeasy Plus Mini kit (QIAGEN, Hilden, Germany)
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA amplified with TargetAmp 1-round Aminoallyl-aRNA Amplication Kit 101 (Epicentre, WI) (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on the human HG-U133 Plus 2.0 GeneChip array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix Genechip Scanner 3000.
Description gene expression data from human colon
Data processing The data were analyzed with GeneSpring version 7.3 using RMA normalization with a custom CDF file(Hs133P_Hs_REFSEQ_8), then normalized to the batch-specific '107' internal control (see Series record for the '107' CEL files)
 
Submission date Nov 26, 2007
Last update date Aug 14, 2011
Contact name Lee Denson
E-mail(s) lee.denson@cchmc.org
Organization name CCHMC
Street address 3333 Burnet Avenue
City Cincinnati
State/province OH
ZIP/Postal code 45229
Country USA
 
Platform ID GPL5760
Series (1)
GSE9686 Human colon expression in healthy, CD, treated CD, and UC

Data table header descriptions
ID_REF
VALUE data was RMA normalized, then normalized to the internal control sample for each batch

Data table
ID_REF VALUE
NM_000014_at 2.0203311
NM_000015_at 0.9440127
NM_000016_at 0.9313144
NM_000017_at 0.508428
NM_000018_at 0.55374306
NM_000019_at 0.7682842
NM_000020_at 1.3380164
NM_000021_at 0.771574
NM_000022_at 1.0186458
NM_000023_at 1.2814931
NM_000024_at 1.489715
NM_000025_at 0.9912908
NM_000026_at 1.0872169
NM_000027_at 1.7067715
NM_000028_at 0.70710593
NM_000029_at 3.4390693
NM_000030_at 0.93845725
NM_000031_at 0.65876937
NM_000032_at 1.0084103
NM_000033_at 1.2637852

Total number of rows: 26121

Table truncated, full table size 596 Kbytes.




Supplementary file Size Download File type/resource
GSM244768.CEL.gz 5.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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