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Status
Public on Sep 19, 2002
Title
B7hi mTEC B6/129 aire-/- 2
Sample type
RNA
Source name
murine medullary thymic epithelial cells
Organism
Mus musculus Extracted molecule
total RNA
Description
Mice used were B6/129 F2's, 3-5 weeks of age, with both copies of the autoimmune regulator gene (aire, GenBank #AF079536) disrupted. Thymi from five of these mice of both sexes were removed and pooled. After collagenase/dispase digestion, density gradient fractionation, and fluorescent antibody staining, cells with the phenotype CD45-, G8.8+, CDR1int and B7.1hi were FACS-sorted and total RNA was made from them. RNA was twice-amplified using a T7 polymerase-based method.
Submission date
Sep 18, 2002
Last update date
May 27, 2005
Contact name
CBDM Lab
E-mail(s)
cbdm@hms.harvard.edu Phone
617-432-7747
Organization name
Harvard Medical School
Department
Microbiology and Immunobiology
Lab
CBDM
Street address
77 Avenue Louis Pasteur
City
Boston
State/province
MA
ZIP/Postal code
02215
Country
USA
Platform ID
GPL81 Series (1)
GSE85 wild type and aire -/- murine meduallary thymic epithelial cells
Data table header descriptions ID_REF VALUE Microarray Suite 5.0 raw signal
ABS_CALL Microarray Suite 5.0 call -- absent, present, marginal
NORM_VALUE signal values after processing with "Norm" software
Data table ID_REF VALUE ABS_CALL NORM_VALUE 100001_at
0.8
A
1
100002_at
0.9
A
1
100003_at
2.3
A
2.1
100004_at
7.4
M
6.18
100005_at
19.7
P
16.23
100006_at
4.9
P
4.24
100007_at
8.2
A
6.8
100009_r_at
9.2
P
7.41
100010_at
0.9
A
1.03
100011_at
23.7
P
18.76
100012_at
460
P
365.42
100013_at
105
P
81.05
100014_at
10.5
P
8.8
100015_at
30.1
P
23.93
100016_at
5.7
A
4.88
100017_at
8.8
M
7.48
100018_at
11
A
9.08
100019_at
6.5
A
5.54
100020_at
35.8
P
28.18
100021_at
3.2
A
2.85
12488 262 Kbytes .Supplementary file Size Download File type/resource GSM2448.CEL.gz
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