|
| Status |
Public on Jan 10, 2017 |
| Title |
Distal blastema 14dpa RA-treated 3 |
| Sample type |
RNA |
| |
|
| Source name |
forelimb
|
| Organism |
Ambystoma mexicanum |
| Characteristics |
size: 8.8cm total length
|
| Treatment protocol |
Animals were anesthetized via treatment of 0.01% benzocaine and forelimbs were amputated at the distal zeugopod. Between days 7-14 dpa, individually housed animals were dosed with RA, LE135, or DMSO (n = 16/treatment). Drugs were changed every other day. Blastemas containing as little stump tissue as possible were collected from all 48 animals at 14 dpa and single forelimbs from four separate individuals were pooled together to yield four independent biological replicate samples for each treatment group.
|
| Growth protocol |
Animals were maintained at 18-20 degrees C individually in salamander housing solution.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted via Qiagen RNeasy kit per manufacturer’s instructions. RNA quality was assessed using an Epoch microplate spectrophotometer, gel electrophoresis, and a 2100 Agilent Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotinylation was carried out according to the instructions in Affymetrix's 3'IVT Reagent Kit user's manual
|
| |
|
| Hybridization protocol |
Affymetrix Gene Chip 3'IVT Reagent Kit was used according to manufacturer's instructions.
|
| Scan protocol |
Affymetrix Command Console was used according to manufacturer's instructions
|
| Description |
Distal blastema 14 days after amputation treated with RA
|
| Data processing |
Expression values were generated using the Robust Microarray Average (RMA) algorithm.
GPL15153_AMBY_002a520748F
|
| |
|
| Submission date |
Jan 09, 2017 |
| Last update date |
Jan 10, 2017 |
| Contact name |
James R Monaghan |
| E-mail(s) |
j.monaghan@northeastern.edu
|
| Organization name |
Northeastern University
|
| Department |
Biology
|
| Lab |
Monaghan Lab
|
| Street address |
360 Huntington Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL15153 |
| Series (1) |
| GSE93303 |
Expression data from regenerating axolotl forelimbs |
|
