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Sample GSM2458520 Query DataSets for GSM2458520
Status Public on Jul 18, 2017
Title LNCaP_vehicle+R1881_rep3
Sample type RNA
Source name LNCaP cells, 48h R1881 treatment
Organism Homo sapiens
Characteristics cell line: human LNCaP prostate cancer cells (immortalized cell line)
: R1881
Treatment protocol Cells were treated for 48h with R1881 (10nM), Torin 1 (100nM), or vehicles (EtOH+DMSO).
Growth protocol Cells were seeded in RPMI media with no phenol red and with charcoal-stripped serum (no steroids) . 48h later, cell were treated with 10nM R1881, Torin 1 (100nM) or vehicles (EtOH and DMSO) for 48h. Triplicate samples were sent for each conditions.
Extracted molecule total RNA
Extraction protocol RNA was purified using RNeasy mini kit from QIAGEN
Label biotin
Label protocol Labeling was performed using standard Illumina protocol at Génome Québec Innovation Centre (McGill University)
Hybridization protocol RNA was hybridized to Illumina HumanHT-12 Expression Beachip v4 and quantified at Génome Québec Innovation Centre (McGill University)
Scan protocol Scan of the microarrays was performed by the Génome Québec Innovation Centre (McGill University)
Description vehicler18813
Data processing The expression data were analyzed using FlexArray10 software.
FlexArray10 software was using for data normalization and expression comparison between groups.
Submission date Jan 13, 2017
Last update date Jul 18, 2017
Contact name Etienne Audet-Walsh
Organization name Université Laval
Department Molecular Medicine
Street address 2705 boulevard Laurier
City Québec City
State/province QC
ZIP/Postal code G1V 4G2
Country Canada
Platform ID GPL10558
Series (2)
GSE93603 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression [array]
GSE93846 Nuclear mTOR acts as a transcriptional integrator of the androgen signaling pathway in prostate cancer progression

Data table header descriptions
VALUE normalized signal

Data table
ILMN_1728255 6.19007884069544
ILMN_1846128 6.18664100973974
ILMN_1651705 8.29328121423192
ILMN_1853167 6.18892568168538
ILMN_1695844 6.18942157525364
ILMN_1874323 6.20283842214061
ILMN_1666310 6.23511201969517
ILMN_1757152 6.24239887558182
ILMN_3236052 6.17211035805753
ILMN_2043569 6.38005661143317
ILMN_1811364 6.19060793124259
ILMN_1719498 6.45867491731178
ILMN_1763663 7.88798952588506
ILMN_1842320 6.19098786341616
ILMN_2278908 12.5588347198951
ILMN_1777359 6.19163192961781
ILMN_1779373 6.22082151065462
ILMN_1800412 6.3392486238881
ILMN_1812441 7.22321545084402
ILMN_1747946 6.19038611334146

Total number of rows: 47323

Table truncated, full table size 1381 Kbytes.

Supplementary data files not provided
Processed data included within Sample table

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