|
| Status |
Public on Apr 03, 2018 |
| Title |
Preadipocyte - 3T3-L1 cell line (VFI03) |
| Sample type |
RNA |
| |
|
| Source name |
Preadipocyte - 3T3-L1 cell line
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Adipose tissue
cell line: 3T3-L1
genotype/variation: KD TP53INP2
time: day 0 of differentiation
cell type: preadipocyte
replicate: 3
|
| Treatment protocol |
Preadipocytes were transduced with lentiviruses encoding scrambled or TP53INP2 siRNA sequences.
|
| Growth protocol |
Preadipocytes were maintained in Proliferation medium.Two day post-confluent (T=0) cell were induced to diferentiate with Differentiation medium until T=7.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using Trizol / RNeasy method (Qiagen RNeasy mini kit (#74104)). Library preparation and amplification were performed using Ovation RNA Amp System V (Nugen;3100-60)
|
| Label |
biotin
|
| Label protocol |
Fragmentation and labelling were performed using Encode Biotin Module (Nugen; 4200-60)
|
| |
|
| Hybridization protocol |
Labeled DNA was hybridized to the microarray for 16 hours at 45ºC, washing and staining of microarrays was performed using a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
Arrays were scanned with GeneChip scanner GSC3000 (Affymetrix, Santa Clara, CA)
|
| Data processing |
RMA normalization method as implemented in affy Bioconductor R package
|
| |
|
| Submission date |
Jan 15, 2017 |
| Last update date |
Apr 03, 2018 |
| Contact name |
Antoni Berenguer-LLergo |
| E-mail(s) |
aberenguerl@tauli.cat
|
| Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
| Department |
A8G2 Group - Rheumatology Service
|
| Street address |
Parc del Taulí, 1
|
| City |
Sabadell |
| State/province |
Barcelona |
| ZIP/Postal code |
08208 |
| Country |
Spain |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE93637 |
TP53INP2 knockdown in 3T3-L1 preadipocytes |
|
