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Status |
Public on Apr 03, 2018 |
Title |
Adipocyte - 3T3-L1 cell line (VF721) |
Sample type |
RNA |
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Source name |
Adipocyte - 3T3-L1 cell line
|
Organism |
Mus musculus |
Characteristics |
tissue: Adipose tissue cell line: 3T3-L1 genotype/variation: Control time: day 7 of differentiation cell type: adipocyte replicate: 1
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Treatment protocol |
Preadipocytes were transduced with lentiviruses encoding scrambled or TP53INP2 siRNA sequences.
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Growth protocol |
Preadipocytes were maintained in Proliferation medium.Two day post-confluent (T=0) cell were induced to diferentiate with Differentiation medium until T=7.
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using Trizol / RNeasy method (Qiagen RNeasy mini kit (#74104)). Library preparation and amplification were performed using Ovation RNA Amp System V (Nugen;3100-60)
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Label |
biotin
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Label protocol |
Fragmentation and labelling were performed using Encode Biotin Module (Nugen; 4200-60)
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Hybridization protocol |
Labeled DNA was hybridized to the microarray for 16 hours at 45ºC, washing and staining of microarrays was performed using a GeneChip Fluidics Station 450 (Affymetrix, Santa Clara, CA)
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Scan protocol |
Arrays were scanned with GeneChip scanner GSC3000 (Affymetrix, Santa Clara, CA)
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Data processing |
RMA normalization method as implemented in affy Bioconductor R package
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Submission date |
Jan 15, 2017 |
Last update date |
Apr 03, 2018 |
Contact name |
Antoni Berenguer-LLergo |
E-mail(s) |
aberenguerl@tauli.cat
|
Organization name |
Parc Taulí Research and Innovation Institute (I3PT)
|
Department |
A8G2 Group - Rheumatology Service
|
Street address |
Parc del Taulí, 1
|
City |
Sabadell |
State/province |
Barcelona |
ZIP/Postal code |
08208 |
Country |
Spain |
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|
Platform ID |
GPL1261 |
Series (1) |
GSE93637 |
TP53INP2 knockdown in 3T3-L1 preadipocytes |
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