|
| Status |
Public on Sep 22, 2008 |
| Title |
Vasectomised epididimus,47 years-old, caput segment |
| Sample type |
RNA |
| |
|
| Source name |
Vasectomised epididimus,47 years-old, caput segment
|
| Organism |
Homo sapiens |
| Characteristics |
Epididymis
|
| Treatment protocol |
Dissection protocol: Tissues were collected under optimum conditions while artificial circulation was maintained to preserve organs assigned for transplantation. Epididymides were dissected in caput, corpus and cauda epididymidis and immediately frozen in liquid nitrogen and stored at -80°C until used for RNA extraction
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol reagent according to manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared accordind to the standard Affymetrix protocol from 10 µg total RNA (Expression Analysis technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentaion, 15 µg of cRNA were hybridized for 16h at 45°C to GeneChip U133 Plus 2.0 Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450 (EukGE-WS2v5_450).
|
| Scan protocol |
GeneChips were scanned using the Affymetrix Scanner 3000 G7 and extracted by GCOS 1.2 software (Affymetrix).
|
| Description |
Gene expression data from Normal epididimus,50 years-old, tail segment
biological rep 3
|
| Data processing |
The data were analyzed with LimmaGUI software (Bioconductor). Data was normalized by RMA method.
|
| |
|
| Submission date |
Nov 29, 2007 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Ezequiel L Calvo |
| E-mail(s) |
cezequiel@yahoo.com
|
| Organization name |
CRCHUL
|
| Department |
Molecular Endocrinilogy
|
| Lab |
Microarrays
|
| Street address |
2705 Boul. Laurier
|
| City |
Quebec |
| State/province |
Quebec |
| ZIP/Postal code |
G1V 4G2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE9731 |
Effects of vasectomy on gene expression profiling along the human epididymis |
|
| Relations |
| Reanalyzed by |
GSE119087 |
