|
| Status |
Public on Feb 02, 2017 |
| Title |
caseine estradiol rep 3 |
| Sample type |
RNA |
| |
|
| Source name |
postnatal day 34 rat testes
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: testes
developmental stage: postnatal day 34
diet: 17-b estradiol
strain: Sprague-Dawley
|
| Treatment protocol |
Testes were collected and fresh frozen in liquid nitrogen
|
| Growth protocol |
Pups of Sprague-Dawley were feed casein AIN-93G diets thorugh lactation. On postnatal day (PND) 21, pups were assigned to one of four diet groups: casein AIN-93G; casein AIN-93G and infused s.c 17beta estradiol at 10 ug/kg/d through mini-osmotic pumps; AIN-93G made with SPI in place of casein; AIN-93G made with SPI and infused s.c. 17beta estradiol. At sacrifice (PND 34) testes were collected and fresh frozen in liquid nitrogen.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from the rats testes using TRI reagent and purified using RNeasy mini columns including on-column Dnase digestion
|
| Label |
biotin
|
| Label protocol |
First- and second- strand cDNA synthesis (from 8 ug total RNA), biotin-labeled aRNA synthesis, fragmentation of cRNA reactions were performed using Affymetrix protocols. aRNA was synthesized from cDNA using a GeneChip IVT labeling kit (Affymetrix) according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
Twenty microgram aRNA was then fragmented in 5X fragmentation buffer for 35 min at 94 °C. aRNAs were hybridized to an individual Affymetrix GeneChip Rat arrays (N = 3/treatment) for 16 h at 45ºC in the hybridization oven set at 60 rpm.
|
| Scan protocol |
The probe array was washed and stained using GeneChip fluidics station 450 and scanned using GeneChip Scanner 3000.
|
| Data processing |
Cel files were generated using GCOS and utilized for analysis using R/Bioconductor 3.4
|
| |
|
| Submission date |
Feb 01, 2017 |
| Last update date |
Feb 02, 2017 |
| Contact name |
Horacio Gomez-Acevedo |
| E-mail(s) |
horacio.g.acevedo@gmail.com
|
| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Biomedical Informatics
|
| Street address |
4301 W. Markham St
|
| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE94392 |
Selective estrogen receptor modulator-like gene expression in prepubertal rat testis after soy feeding |
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