|
| Status |
Public on Sep 11, 2020 |
| Title |
GFPlow_M9 |
| Sample type |
RNA |
| |
|
| Source name |
Crypt cells expressing low levels of GFP, early Flattop lineage, RFP-negative
|
| Organism |
Mus musculus |
| Characteristics |
tissue: small intestinal crypts
strain: mixed C57BL/6J, CD1, 129/SvJ
age: 3-6 months
genotype: FltpT2AiCre crossed with Gt(ROSA)26mTmG
|
| Treatment protocol |
Small intestinal crypts were isolated from FltpZV/+ mice. Single cells were pepared and FAC-sorted according to the GFP level.
|
| Growth protocol |
No growth
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRNeasy Mini Kit (Qiagen) including DNase treatment
|
| Label |
biotin
|
| Label protocol |
Ovation Pico WTA System V2 in combination with the Encore Biotin Module (Nugen)
|
| |
|
| Hybridization protocol |
According to the Affymetrix expression protocol
|
| Scan protocol |
According to the Affymetrix expression protocol, Scanner 3000 7G
|
| Description |
Gene expression data from crypt cells expressing low levels of GFP, early Flattop lineage, RFP negative
|
| Data processing |
Expression console (v.1.3.0.187, Affymetrix) was used to obtain normalized RMA gene-level data (standard settings including median polish and sketch-quantile normalisation). The 18 samples have been normalized together but RMA data provided for only 6 samples.
|
| |
|
| Submission date |
Feb 02, 2017 |
| Last update date |
Sep 11, 2020 |
| Contact name |
Johannes Beckers |
| E-mail(s) |
johannes.beckers@helmholtz-munich.de
|
| Organization name |
Helmholtz Zentrum Muenchen
|
| Department |
Institute of Experimental Genetics
|
| Street address |
Ingolstaedter Landstr. 1
|
| City |
Neuherberg |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE72112 |
Wnt/planar cell polarity primed intestinal stem cells directly differentiate into enteroendocrine or Paneth cells |
| GSE94092 |
Non-canonical Wnt/PCP signalling regulates intestinal stem cell lineage priming towards enteroendocrine and Paneth cell fates |
|
