|
| Status |
Public on Dec 01, 2017 |
| Title |
9-C2C miRNA |
| Sample type |
RNA |
| |
|
| Source name |
SH-SY5Y cells Cytoplasm, biological replicate 2
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: undifferentiated SH-SY5Y cells
|
| Treatment protocol |
Nucleus and Cytoplasm were fractionated by hypotonic lysis.
|
| Growth protocol |
Cells were grown in high-glucose DMEM with 10% FCS under standard conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol extraction with addition of 2ul glycogen during precipitation step, and overnight precipitation.
|
| Label |
biotin
|
| Label protocol |
miRNA HSR FlashTag Biotin labelling kit (Genisphere)
|
| |
|
| Hybridization protocol |
Standard Affymetrix hybridisation protocol.
|
| Scan protocol |
Standard Affymetrix scan protocol.
|
| Description |
miRNA expression data from undifferentiated SH-SY5Y cells
|
| Data processing |
Raw expression data were exported from Genespring GX for further analysis in Excel.
|
| |
|
| Submission date |
Feb 03, 2017 |
| Last update date |
Dec 01, 2017 |
| Contact name |
Murray J Cairns |
| E-mail(s) |
murray.cairns@newcastle.edu.au
|
| Organization name |
University of Newcastle
|
| Street address |
University Drive
|
| City |
Callaghan |
| State/province |
NSW |
| ZIP/Postal code |
2307 |
| Country |
Australia |
| |
|
| Platform ID |
GPL8786 |
| Series (2) |
| GSE94483 |
miRNA expression data from fractionated SH-SY5Y cells |
| GSE94485 |
miRNA & mRNA expression data from fractionated SH-SY5Y cells |
|
