|
| Status |
Public on Nov 01, 2017 |
| Title |
LPS activated bovine macrophages stimulated with FhWild-ES, replicate 3 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
BOMA, bovine macrophage cell line
|
| Organism |
Bos taurus |
| Characteristics |
cell line: BOMA
cell type: monocytes/ macrophages
agent: none
|
| Treatment protocol |
Cells were ativated with LPS (8.9 µg/ml) and stimulated in quadruplicate with FhWey-ES (10 µg/ml for 26 hours) or FhWild-ES (10 µg/ml for 24 hours)
|
| Growth protocol |
The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using RNA isolation kit (A&A Biotechnology)
|
| Label |
Cy3
|
| Label protocol |
Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
|
| |
|
| Channel 2 |
| Source name |
BOMA, bovine macrophage cell line stimulated with FhWild-ESP
|
| Organism |
Bos taurus |
| Characteristics |
agent: FhWild-ES
cell line: BOMA
cell type: monocytes/ macrophages
|
| Treatment protocol |
Cells were ativated with LPS (8.9 µg/ml) and stimulated in quadruplicate with FhWey-ES (10 µg/ml for 26 hours) or FhWild-ES (10 µg/ml for 24 hours)
|
| Growth protocol |
The cells were cultivated in DMEM (with 5% FCS, 2 mM glutamine, 100 units/ml of penicillin, 100 µg/ml streptomycin)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted using RNA isolation kit (A&A Biotechnology)
|
| Label |
Cy5
|
| Label protocol |
Quick Amp Labeling (Agilent Technologies) according to the manufacturer`s protocol
|
| |
|
| |
| Hybridization protocol |
according to the manufacturer's protocol
|
| Scan protocol |
Scanned on an Agilent G2505C scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
| Description |
Technical replicate 3 of 4
|
| Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
| |
|
| Submission date |
Feb 17, 2017 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Piotr Bąska |
| E-mail(s) |
piotr_baska@sggw.edu.pl
|
| Phone |
48225936038
|
| Organization name |
Warsaw University of Life Sciences WULS – SGGW
|
| Department |
Department of Preclinical Sciences
|
| Lab |
Division of Pharmacology and Toxicology
|
| Street address |
Ciszewskiego 8
|
| City |
Warsaw |
| State/province |
Mazowieckie |
| ZIP/Postal code |
02-786 |
| Country |
Poland |
| |
|
| Platform ID |
GPL11648 |
| Series (1) |
| GSE95022 |
Analysis of influence of Excretory Secretory Products from wild and laboratory Fasciola hepatica isolates on LPS activated bovine macrophages |
|
