|
Status |
Public on Oct 23, 2021 |
Title |
LB10 |
Sample type |
SRA |
|
|
Source name |
Gossypium hirsutum 10 DPA
|
Organism |
Gossypium hirsutum |
Characteristics |
developmental stage: 10 DPA tissue: fiber
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was treated with RQ1 DNase (Promega) to remove DNA. The quality and quantity of the purified RNA were determined by measuring the absorbance at A260/A280 and A260/A230 using smartspec plus (BioRad) RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Data processing |
Illumina Casava1.5 software used for basecalling. 3' adapter(TTAGATCAG) trimmed with cutadapt -e 0.12 -O 6 --trimmed-only -m 15 -M 25 mapped using bowtie2 -t -N 1 -p 24 detecting different expression gene using edgeR Genome_build: Gossypium_hirsutum_v1.1 Supplementary_files_format_and_content: tab-delimited text files include gene RPKM values for each Sample
|
|
|
Submission date |
Feb 21, 2017 |
Last update date |
Oct 23, 2021 |
Contact name |
dong chen |
Organization name |
ablife
|
Street address |
GaoXin 2nd Road
|
City |
wuhan |
State/province |
Hubei |
ZIP/Postal code |
430064 |
Country |
China |
|
|
Platform ID |
GPL16485 |
Series (1) |
GSE95146 |
A genome-wide analysis of expression differences during fiber initiation between Gossypium hirsutum and Gossypium barbadense |
|
Relations |
BioSample |
SAMN06361560 |
SRA |
SRX2581069 |