|
| Status |
Public on May 08, 2017 |
| Title |
T47D_vehicle_Rep3 |
| Sample type |
RNA |
| |
|
| Source name |
T47D vehicle
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T47D
agent: vehicle
|
| Treatment protocol |
Cells were treated with 1uM JQ1 or vehicle (DMSO) and incubated for 24 hours at 37ºC in a humidified incubator with 5%
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Maxwell ® RSC simply RNA Cell Kit (Promega) following the manufacturer's recommendations
|
| Label |
Cy3
|
| Label protocol |
The RNA samples were first reverse transcribed into cDNA, and these cDNA samples were then labeled using Agilent protocol.
|
| |
|
| Hybridization protocol |
Hybridization, washing and staining were performed using Agilent system instruments and protocols.
|
| Scan protocol |
After washing, microarrays were scanned using an Agilent microarray scanner (Agilent Technologies).
|
| Description |
Gene expression after vehicle (DMSO) treatment 24h
Cell line, T47D vehicle, replicate 3
|
| Data processing |
Intensity values of each scanned feature were quantified using Agilent Feature Extraction Software. Background correction and quantile array normalization was performed using limma library in R software.
|
| |
|
| Submission date |
Feb 23, 2017 |
| Last update date |
May 09, 2017 |
| Contact name |
Manuel Castro |
| E-mail(s) |
mcastrod@idibell.cat
|
| Organization name |
IDIBELL
|
| Department |
PEBC
|
| Lab |
Cancer Epigenetics
|
| Street address |
Av. Gran Vía de l'Hospitalet 199
|
| City |
L'Hospitalet de Llobregat |
| State/province |
Barcelona |
| ZIP/Postal code |
08908 |
| Country |
Spain |
| |
|
| Platform ID |
GPL20844 |
| Series (1) |
| GSE95287 |
Expression profile of MCF7 and T47D human breast cancer cell lines upon treatment with the BET bromodomian inhibitor JQ1 |
|
