|
| Status |
Public on Apr 07, 2008 |
| Title |
PAO1 in MMP supplemented with glutamate and agmatine by Lu |
| Sample type |
RNA |
| |
|
| Source name |
Aerobic batch cultures of P. aeruginosa wild type PAO1
|
| Organism |
Pseudomonas aeruginosa |
| Characteristics |
Wild type PAO1 strain cultured in MMP supplemented with glutamate and agmatine by Lu
|
| Biomaterial provider |
Dr Chung-Dar Lu laboratory, Department of Biology, GSU
|
| Treatment protocol |
P. aeruginosa PAO1 was grown in minimal medium P in the presence of L-glutamate with the addition of agmatine at 20 mM.
|
| Growth protocol |
P. aeruginosa PAO1 was grown aerobically in minimal medium P with 350 rpm shaking at 37C. Cells were harvested when the optical density at 600 nm reached 0.5~0.6 by centrifugation for 5 minutes at 4C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were isolated by RNeasy purification kit following instructions of the manufacturer (Qiagen). Reverse transcription for cDNA synthesis and fragmentation by DNase I treatment were performed according to the instructions of GeneChip manufacturer (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix procedures
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
wild type PAO1
|
| Data processing |
Affymetrix Microarray Suite version 5.0. Chip target intensity is normalized to 500.
|
| |
|
| Submission date |
Dec 17, 2007 |
| Last update date |
Apr 25, 2011 |
| Contact name |
Han Ting Chou |
| Organization name |
Georgia State University
|
| Department |
Biology
|
| Street address |
1 University Park Drive
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30303 |
| Country |
USA |
| |
|
| Platform ID |
GPL84 |
| Series (1) |
| GSE9926 |
Transcriptome Analysis of Agmatine and Putrescine Catabolism in Pseudomonas aeruginosa PAO1 |
|
