|
| Status |
Public on Mar 07, 2017 |
| Title |
uterus_3-MC,rat-2 |
| Sample type |
RNA |
| |
|
| Source name |
rat uterus, 72 h treatment, 3-MC
|
| Organism |
Rattus norvegicus |
| Characteristics |
treatment: 3-MC
tissue: uterus
gender: female
strain: Lewis
|
| Treatment protocol |
Rats were treated with vehicle control (DMSO), 4µg/kg/d 17β-Estradiol (E2), 3-Methylcholanthrene (3-MC) and E2+3-MC for three consecutive days. Uteri were obtained 24 h after the last treatment. Biological samples from three rats were analyzed for each treatment group.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Gene 1.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Scanner 3000 7G with AGCC software.
|
| Description |
ovx_rat_T3-2
|
| Data processing |
CEL files were analyzed for quality using Affymetrix Expression Console software, and imported into GeneSpring GX11.5. Data was quantile-normalized using PLIER and baseline transformed to the median of the control samples.
|
| |
|
| Submission date |
Mar 07, 2017 |
| Last update date |
Mar 07, 2017 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
schittur@albany.edu
|
| Phone |
518-591-7215
|
| Organization name |
SUNY-University at Albany
|
| Department |
Center for Functional Genomics
|
| Lab |
Microarray Core
|
| Street address |
One Discovery Drive, CRC 342G
|
| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
| |
|
| Platform ID |
GPL6247 |
| Series (1) |
| GSE95783 |
Expression data from the uterus of ovariectomized young adult rats treated for three days with E2, 3-MC, E2+3-MC |
|
