|
| Status |
Public on Nov 01, 2017 |
| Title |
Aorta_Preeclampsia_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Aorta, preeclampsia
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Wistar
tissue: Abdominal aorta
gestational age: day 20
infusion: LPS
|
| Treatment protocol |
A cannula was placed into the right jugular vein in animals at day 0 of pregnancy and also in age matched non-pregnant control animals while anesthetized with isoflurane/oxygen. Animals were infused with either LPS or saline 14 days after cannula placement. The infusion took place during 1 hour with 1 μg/kg bodyweight LPS dissolved in 2 ml saline and resulted in experimental preeclampsia. The healthy pregnant control animals received saline only (2 ml during 1h). At day 20 of pregnancy, the animals were terminated by decapitation and thoracic and abdominal aortas were isolated. From non-pregnant female rats with saline infusion aortas were also isolated. The aortas were placed in cold oxygenated Krebs solution and stored at -80°C until further use.
|
| Growth protocol |
Wistar outbred rats (Harlan Inc, Horst, the Netherlands) were housed in a 12-hour light-dark cycle with food and water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from whole abdominal aortas with the use of TriReagent following the manufacturer’s instructions. An additional round of purification was performed with RNeasy Microkit columns. RNA quality was assessed using RNA 6000 nanochips on the Agilent 2100 bioanalyzer.
|
| Label |
Biotin
|
| Label protocol |
Total RNA (100 ng) was labelled using the Affymetrix WT plus reagent kit
|
| |
|
| Hybridization protocol |
Sample hybridization to chipsperformed according to the manufacturer's instructions.
|
| Scan protocol |
Sample image scanning was performed according to the manufacturer's instructions.
|
| Description |
Gene expression data from the aorta from preeclamptic rat
|
| Data processing |
Microarray analysis was performed using MADMAX pipeline for statistical analysis of microarray data. Quality control was performed and all arrays met our criteria. For further analysis a custom annotation was used based on reorganized oligonucleotide probes, which combines all individual probes for a gene (version 19.0.0: http://mbni.org/customcdf/19.0.0/entrezg.download/ragene11st_Rn_ENTREZG_19.0.0.zip). Only genes that had at least 5 probes present on the array were taken into account. Expression values were calculated using robust multichip average (RMA) method, which includes quantile normalisation. Significant differences in expression were assessed using paired Intensity-Based Moderated T-statistic (IBMT)
|
| |
|
| Submission date |
Mar 14, 2017 |
| Last update date |
Nov 01, 2017 |
| Contact name |
Marijke Faas |
| E-mail(s) |
m.m.faas@umcg.nl
|
| Organization name |
University Medical Center Groningen
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL23181 |
| Series (1) |
| GSE96610 |
Gene expression data in aortic tissue from rats with experimental preeclampsia, healthy pregnancy and non pregnant rats |
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