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| Status |
Public on Mar 16, 2017 |
| Title |
N-4507906 |
| Sample type |
RNA |
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| Source name |
Serum
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| Organism |
Homo sapiens |
| Characteristics |
gender: Male
disease: No-atherosclerosis
tissue: Serum
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| Treatment protocol |
n/a
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| Growth protocol |
n/a
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| Extracted molecule |
total RNA |
| Extraction protocol |
TaqMan® miRNA ABC Purification Kit-Human-Panel A/B (part nos. 4473087 and 4473088, Applied Biosystems, Foster City, CA, USA)
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| Label |
FAM
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| Label protocol |
RT: Megaplex™ RT Human Pool A [part no. 4399966] for Panel A and Pool B [part no. 4399968] for Panel B, Applied Biosystems and TaqMan® MicroRNA Reverse Transcription Kit, Part No. 4366596 ; qPCR: TaqMan® Array Human microRNA Cards A and B, Part No. 4444913, Applied Biosystems
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| Hybridization protocol |
n/a
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| Scan protocol |
n/a
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| Data processing |
The normalization and all the data analysis were performed according to the manufacturers instructions using ExpressionSuite for QuantStudio™ 6 & 7 Flex Real Time PCR System
Matrix normalized worksheet reports normalized signal (against endogenous control genes, miR-197 and miR-374).
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| Submission date |
Mar 15, 2017 |
| Last update date |
Mar 16, 2017 |
| Contact name |
Jei Kim |
| E-mail(s) |
jeikim@cnu.ac.kr
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| Phone |
82-42-280-7805
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| Organization name |
Chungnam National University Hospital
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| Street address |
282 Moonhwa-ro, Joong-gu
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| City |
Daejeon |
| ZIP/Postal code |
35015 |
| Country |
South Korea |
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| Platform ID |
GPL18942 |
| Series (1) |
| GSE96621 |
Comparison miRNA expression between atherosclerotic and non-atherosclerotic ischemic stroke patients |
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| Supplementary data files not provided |
| Processed data are available on Series record |
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