|
| Status |
Public on Jan 01, 2008 |
| Title |
2A12_P1_N_EX1_POS |
| Sample type |
genomic |
| |
|
| Source name |
pooled DNA for CTL-2A12 reactive clones
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled DNA sample of 44 human B-LCLs that show positive reactivity for CTL-2A12.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA were extracted using conventional methods. DNA concentrations were measured and adjusted to 50 μg/ml using the PicoGreen dsDNA Quantitation Reagent (Molecular Probes, Eugene, OR).
|
| Label |
biotin
|
| Label protocol |
Biotinylated genomic DNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 microg of DNA were hybridized for 16 hr on GeneChip Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000 7G.
|
| Description |
Compared with pooled sample from CTL negative clones.
|
| Data processing |
genotype-calling Dynamic Model algorithm 3.0; CNAG software.
|
| |
|
| Submission date |
Dec 30, 2007 |
| Last update date |
Dec 31, 2007 |
| Contact name |
Yasuhito Nannya |
| E-mail(s) |
ynanya-tky@umin.ac.jp
|
| Phone |
+81-3-3815-5411
|
| Organization name |
Tokyo University
|
| Department |
Department of Hematology/Oncology
|
| Lab |
Genome Laboratory
|
| Street address |
BUnkyo-ku Hongo 7-3-1
|
| City |
Tokyo |
| ZIP/Postal code |
113-8655 |
| Country |
Japan |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE10044 |
Identification of human minor H antigens based on genotyping of pooled DNA |
|
