NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM254150 Query DataSets for GSM254150
Status Public on Jun 17, 2008
Title small airways, non-smoker 059
Sample type RNA
 
Source name airway epithelial cells obtained by bronchoscopy and brushing
Organism Homo sapiens
Characteristics age: 35
sex: M
ethnic group: black
smoking status: non-smoker
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 3 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description small airways, non-smoker 059
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Jan 03, 2008
Last update date Aug 28, 2018
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (13)
GSE8545 Variability in Small Airway Epithelial Gene Expression Among Normal Smokers
GSE11784 Biologic Phenotyping of the Human Small Airway Epithelial Response to Cigarette Smoking
GSE11906 Quality Control in Microarray Assessment of Gene Expression in Human Airway Epithelium
Relations
Reanalyzed by GSE60486
Reanalyzed by GSE119087

Data table header descriptions
ID_REF
VALUE MAS5-calculated signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 597.807 P 0.000258358
AFFX-BioB-M_at 1496.46 P 4.42873e-05
AFFX-BioB-3_at 739.486 P 5.16732e-05
AFFX-BioC-5_at 2478.72 P 4.42873e-05
AFFX-BioC-3_at 3322.31 P 4.42873e-05
AFFX-BioDn-5_at 4968.87 P 4.42873e-05
AFFX-BioDn-3_at 10022.4 P 4.42873e-05
AFFX-CreX-5_at 29103.3 P 5.16732e-05
AFFX-CreX-3_at 38829.5 P 4.42873e-05
AFFX-DapX-5_at 28.3682 A 0.340661
AFFX-DapX-M_at 31.5318 A 0.58862
AFFX-DapX-3_at 21.3277 A 0.686292
AFFX-LysX-5_at 44.4672 A 0.250796
AFFX-LysX-M_at 10.087 A 0.945787
AFFX-LysX-3_at 46.3881 M 0.0629293
AFFX-PheX-5_at 25.8034 A 0.672935
AFFX-PheX-M_at 11.0967 A 0.852061
AFFX-PheX-3_at 49.3828 A 0.559354
AFFX-ThrX-5_at 4.37473 A 0.987453
AFFX-ThrX-M_at 13.8269 A 0.672921

Total number of rows: 54675

Table truncated, full table size 1636 Kbytes.




Supplementary file Size Download File type/resource
GSM254150.CEL.gz 8.1 Mb (ftp)(http) CEL
GSM254150.CHP.gz 13.4 Mb (ftp)(http) CHP
Processed data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap