|
| Status |
Public on Jul 17, 2017 |
| Title |
Left testis, long sperm line male 2011.M.832 |
| Sample type |
RNA |
| |
|
| Source name |
Zebra finch left testis, long sperm line male 2011.M.832
|
| Organism |
Taeniopygia guttata |
| Characteristics |
tissue: testis
gender: male
age: adult
|
| Treatment protocol |
Testes were treated with RNAlater and stored at -80C until RNA extraction
|
| Growth protocol |
Male zebra finches were euthanized and dissected. Testes were extracted from both sides and washed with PBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA were hybridized on Zebra finch Gene 1.1 ST Array according to the manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan.
|
| Description |
Gene expression data from left testis of a long sperm line male
|
| Data processing |
The data were analyzed with Affymetrix Expression Console ver 1.3 using default analysis settings and RMA (robust multi-array average) as normalization method.
|
| |
|
| Submission date |
Mar 23, 2017 |
| Last update date |
Jul 17, 2017 |
| Contact name |
Kang-Wook Kim |
| Organization name |
The University of Sheffield
|
| Department |
Animal and Plant Sciences
|
| Street address |
Western Bank
|
| City |
Sheffield |
| ZIP/Postal code |
S10 2TN |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL23222 |
| Series (1) |
| GSE96970 |
Expression data from testes of zebra finch males artificially selected for long or short sperm |
|
