|
| Status |
Public on Mar 28, 2017 |
| Title |
Adjacent normal tissue from patient 15 |
| Sample type |
RNA |
| |
|
| Source name |
Adjacent normal tissue from patient 15
|
| Organism |
Homo sapiens |
| Characteristics |
subject status/id: esophageal squamous cell carcinoma (ESCC) patient 15
tissue: normal esophageal tissue
|
| Treatment protocol |
collect pairs of ESCC tissues and adjacent nontumor tisssues
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit. This kit can be used to label any RNA sample, including total RNA, severely degraded RNA, plant RNA, and low molecular weight RNA.
|
| |
|
| Hybridization protocol |
The labled RNA were hybridized for 16 hr at 48C on Affymetrix miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
| Description |
N15
|
| Data processing |
The data were analyzed with miRNA QCTool using Affymetrix default analysis settings and quantile as normalization method.
|
| |
|
| Submission date |
Mar 27, 2017 |
| Last update date |
Mar 28, 2017 |
| Contact name |
chenyu lin |
| E-mail(s) |
linchenyu0427@163.com
|
| Phone |
08615105603949
|
| Organization name |
China Pharmaceutical University
|
| Department |
Life science
|
| Lab |
Hanmei Xu
|
| Street address |
Longmian Road No.639
|
| City |
Nanjing |
| ZIP/Postal code |
211100 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (2) |
| GSE97049 |
A Long Noncoding RNA TTN-AS1 Controls Esophageal Squamous Cell Carcinomas by Functioning as a Competing Endogenous RNA and Scaffolding mRNA Stabilizing Protein HuR [Affymetrix] |
| GSE97051 |
lncRNA-miRNA-mRNA expression profiles in esophageal squamous carcinoma tissues and adjacent normal tissues |
|
