|
Status |
Public on Mar 28, 2017 |
Title |
Adjacent normal tissue from patient 19 |
Sample type |
RNA |
|
|
Source name |
Adjacent normal tissue from patient 19
|
Organism |
Homo sapiens |
Characteristics |
subject status/id: esophageal squamous cell carcinoma (ESCC) patient 19 tissue: normal esophageal tissue
|
Treatment protocol |
collect pairs of ESCC tissues and adjacent nontumor tisssues
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
About 1ug total RNA were polyA tailed and labled with FlashTag™ Biotin HSR Labeling Kit. This kit can be used to label any RNA sample, including total RNA, severely degraded RNA, plant RNA, and low molecular weight RNA.
|
|
|
Hybridization protocol |
The labled RNA were hybridized for 16 hr at 48C on Affymetrix miRNA Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip® Scanner 3000.
|
Description |
N19
|
Data processing |
The data were analyzed with miRNA QCTool using Affymetrix default analysis settings and quantile as normalization method.
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|
|
Submission date |
Mar 27, 2017 |
Last update date |
Mar 28, 2017 |
Contact name |
chenyu lin |
E-mail(s) |
linchenyu0427@163.com
|
Phone |
08615105603949
|
Organization name |
China Pharmaceutical University
|
Department |
Life science
|
Lab |
Hanmei Xu
|
Street address |
Longmian Road No.639
|
City |
Nanjing |
ZIP/Postal code |
211100 |
Country |
China |
|
|
Platform ID |
GPL21572 |
Series (2) |
GSE97049 |
A Long Noncoding RNA TTN-AS1 Controls Esophageal Squamous Cell Carcinomas by Functioning as a Competing Endogenous RNA and Scaffolding mRNA Stabilizing Protein HuR [Affymetrix] |
GSE97051 |
lncRNA-miRNA-mRNA expression profiles in esophageal squamous carcinoma tissues and adjacent normal tissues |
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